Phelios AL

Luminometer I Multi-mode microplate AB-2300 Phelios AL: A Versatile Tool for Luminescence and Absorbance Measurement

The Luminometer I Multi-mode microplate AB-2300 Phelios AL is a powerful and versatile instrument designed for luminescence and absorbance measurements in various research applications. This compact and user-friendly microplate reader offers a wide range of features, making it an invaluable tool for scientists working in diverse fields, including:

Features:

• Multi-mode detection: Measures both luminescence and absorbance, providing a comprehensive platform for various assays.
• High sensitivity: Detects even the faintest luminescent signals, enabling sensitive and accurate measurements.
• Broad wavelength range: Covers a wide range of wavelengths (200 to 999 nm) for absorbance measurements, catering to a variety of applications.
• Multiple plate formats: Compatible with various plate formats, including 6/12/24/48/96/384-well plates, accommodating a wide range of sample sizes.
• User-friendly software: Intuitive and easy-to-use software simplifies data acquisition and analysis.

Applications:

• Enzyme-linked immunosorbent assays (ELISAs): Quantifies the presence of antigens or antibodies in biological samples.
• Reporter gene assays: Measures gene expression levels using luminescent or fluorescent reporters.
• Kinetics studies: Monitors the rate of enzymatic reactions or other time-dependent processes.
• Protein assays: Quantifies protein concentrations using various colorimetric or fluorometric methods.
• Nucleic acid assays: Measures DNA or RNA concentrations using absorbance or fluorescence-based methods.

Specifications:

• Luminescence sensitivity: 1.0 pg ATP
• Absorbance range: 200 to 999 nm
• Wavelength accuracy: ±1.0 nm
• Read speed: Up to 100 plates per hour
• Dimensions: 345 × 360 × 180 mm
• Weight: 10 kg

Additional Features:

• NanoVolume Plate (NVP) compatibility: Measures microvolume samples (2 µL) for enhanced sensitivity and sample conservation.
• Quantitative analysis mode: Automatically performs calibration curve creation and concentration conversion for quantitative measurements.
• Data export capabilities: Exports data in various formats for further analysis and reporting.

Overall, the Luminometer I Multi-mode microplate AB-2300 Phelios AL is a valuable tool for researchers seeking a versatile and reliable microplate reader for luminescence and absorbance measurements. Its comprehensive features, user-friendly software, and wide range of applications make it an ideal choice for diverse research settings.

Absorbance -ABS-

Wavelength Measurement Range: 200-999nm

• Semi-automated calibration curve creation
• Endpoint measurement
• Kinetic measurement
• Spectral measurement
• Well-area scan measurement
• Capable of measuring up to 6 wavelengths

Phelios AL Luminescence Measurement Capabilities:

1. Photomultiplier Tube (PMT) Technology:

• Phelios AL is equipped with a PMT, a highly sensitive and versatile detector for luminescence measurements. The PMT technology ensures accurate and reliable detection of low-level signals.

2. Low Background Noise and Minimal Crosstalk:

• The instrument excels in providing a low background noise environment, enhancing the signal-to-noise ratio for precise measurements.
• Minimal crosstalk ensures that the luminescence signal from one well does not interfere with adjacent wells, maintaining data accuracy.

3. Wide Dynamic Measurements:

• Phelios AL supports wide dynamic measurements, allowing the detection of a broad range of luminescence intensities. This capability is essential for accommodating diverse experimental conditions.

4. Exceptional Detection Limit:

• With an impressive detection limit of 1 × 10-18 ATP, Phelios AL can detect extremely low levels of luminescence, making it suitable for applications requiring high sensitivity.

5. Dynamic Range of 8 Digits:

• The instrument offers a dynamic range of 8 digits, providing the ability to measure a wide span of luminescence intensities within a single experiment.

6. Luminescence Measurement Modes:

• Endpoint Measurement: This mode allows for the capture of luminescence signals at a specific time point, providing a snapshot of the reaction.
• Kinetics Measurement: This mode captures the luminescence signal over a defined period, enabling the monitoring of dynamic changes in the reaction over time.

7. Applications:

• Phelios AL is well-suited for a variety of applications in molecular biology, biochemistry, and cell biology.
• It is particularly valuable for assays involving bioluminescence, chemiluminescence, and other luminescent reactions.

8. User-Friendly Interface:

• The instrument is designed with a user-friendly interface, making it accessible for both novice and experienced researchers.
• It offers intuitive controls for setting up and conducting luminescence experiments.

Phelios AL, with its advanced features and capabilities, provides researchers with a powerful tool for accurate and sensitive luminescence measurements across a wide range of applications.