Phelios AL

ABS/LUMI/NANO Multimode Microplate reader

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AB-2300 Phelios AL

Phelios AL is a plate reader capable of absorbance measurement in the wavelength range of 200-999 nm and luminescent measurement in 300-700 nm. Nano Volume Plate (optional) enables to measure the samples of only 2 μL.
Embrace precision and efficiency with Phelios AL, the compact and versatile multi-mode microplate reader that empowers you to conduct a wide range of luminescence and absorbance assays. This innovative instrument seamlessly integrates into your laboratory workflow, offering exceptional sensitivity, a broad wavelength range, and compatibility with various plate formats.

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Luminometer I Multi-mode microplate AB-2300 Phelios AL: A Versatile Tool for Luminescence and Absorbance Measurement

The Luminometer I Multi-mode microplate AB-2300 Phelios AL is a powerful and versatile instrument designed for luminescence and absorbance measurements in various research applications. This compact and user-friendly microplate reader offers a wide range of features, making it an invaluable tool for scientists working in diverse fields, including:


  • Multi-mode detection: Measures both luminescence and absorbance, providing a comprehensive platform for various assays.
  • High sensitivity: Detects even the faintest luminescent signals, enabling sensitive and accurate measurements.
  • Broad wavelength range: Covers a wide range of wavelengths (200 to 999 nm) for absorbance measurements, catering to a variety of applications.
  • Multiple plate formats: Compatible with various plate formats, including 6/12/24/48/96/384-well plates, accommodating a wide range of sample sizes.
  • User-friendly software: Intuitive and easy-to-use software simplifies data acquisition and analysis.


  • Enzyme-linked immunosorbent assays (ELISAs): Quantifies the presence of antigens or antibodies in biological samples.
  • Reporter gene assays: Measures gene expression levels using luminescent or fluorescent reporters.
  • Kinetics studies: Monitors the rate of enzymatic reactions or other time-dependent processes.
  • Protein assays: Quantifies protein concentrations using various colorimetric or fluorometric methods.
  • Nucleic acid assays: Measures DNA or RNA concentrations using absorbance or fluorescence-based methods.


  • Luminescence sensitivity: 1.0 pg ATP
  • Absorbance range: 200 to 999 nm
  • Wavelength accuracy: ±1.0 nm
  • Read speed: Up to 100 plates per hour
  • Dimensions: 345 × 360 × 180 mm
  • Weight: 10 kg

Additional Features:

  • NanoVolume Plate (NVP) compatibility: Measures microvolume samples (2 µL) for enhanced sensitivity and sample conservation.
  • Quantitative analysis mode: Automatically performs calibration curve creation and concentration conversion for quantitative measurements.
  • Data export capabilities: Exports data in various formats for further analysis and reporting.

Overall, the Luminometer I Multi-mode microplate AB-2300 Phelios AL is a valuable tool for researchers seeking a versatile and reliable microplate reader for luminescence and absorbance measurements. Its comprehensive features, user-friendly software, and wide range of applications make it an ideal choice for diverse research settings.

Plate Types Available

Phelios AL is compatible with a range of microplate types. When conducting measurements, please choose from the predefined list of plates to initiate the measurement process.

Nano Volume Plate(Option)

Nano Volume Plate (Option) Designed specifically for measuring the absorbance of micro-volume samples ranging from 2 to 5 µL. Ideal for concentration determination of nucleic acids and proteins, as well as calculating DNA/RNA ratios.

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Absorbance -ABS-

Wavelength Measurement Range: 200-999nm

  • Semi-automated calibration curve creation
  • Endpoint measurement
  • Kinetic measurement
  • Spectral measurement
  • Well-area scan measurement
  • Capable of measuring up to 6 wavelengths

Luminosity - LUMI  

Phelios AL comes standard with a PMT (photomultiplier tube), allowing for precise luminescence measurements. It excels in low background noise, minimizes crosstalk, and supports wide dynamic measurements. With a detection limit as low as 1 × 10-18 ATP, it offers an impressive dynamic range of 8 digits. Luminescence measurements include options for both 'Endpoint' and 'Kinetics' measurements.

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Phelios AL Luminescence Measurement Capabilities:

1. Photomultiplier Tube (PMT) Technology:

  • Phelios AL is equipped with a PMT, a highly sensitive and versatile detector for luminescence measurements. The PMT technology ensures accurate and reliable detection of low-level signals.

2. Low Background Noise and Minimal Crosstalk:

  • The instrument excels in providing a low background noise environment, enhancing the signal-to-noise ratio for precise measurements.
  • Minimal crosstalk ensures that the luminescence signal from one well does not interfere with adjacent wells, maintaining data accuracy.

3. Wide Dynamic Measurements:

  • Phelios AL supports wide dynamic measurements, allowing the detection of a broad range of luminescence intensities. This capability is essential for accommodating diverse experimental conditions.

4. Exceptional Detection Limit:

  • With an impressive detection limit of 1 × 10-18 ATP, Phelios AL can detect extremely low levels of luminescence, making it suitable for applications requiring high sensitivity.

5. Dynamic Range of 8 Digits:

  • The instrument offers a dynamic range of 8 digits, providing the ability to measure a wide span of luminescence intensities within a single experiment.

6. Luminescence Measurement Modes:

  • Endpoint Measurement: This mode allows for the capture of luminescence signals at a specific time point, providing a snapshot of the reaction.
  • Kinetics Measurement: This mode captures the luminescence signal over a defined period, enabling the monitoring of dynamic changes in the reaction over time.

7. Applications:

  • Phelios AL is well-suited for a variety of applications in molecular biology, biochemistry, and cell biology.
  • It is particularly valuable for assays involving bioluminescence, chemiluminescence, and other luminescent reactions.

8. User-Friendly Interface:

  • The instrument is designed with a user-friendly interface, making it accessible for both novice and experienced researchers.
  • It offers intuitive controls for setting up and conducting luminescence experiments.

Phelios AL, with its advanced features and capabilities, provides researchers with a powerful tool for accurate and sensitive luminescence measurements across a wide range of applications.